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KMID : 1234920200200020078
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Journal of Korean Oriental Association for Study of Obesity
2020 Volume.20 No. 2 p.78 ~ p.87
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Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions
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Choi Hye-Min
Yu Byung-Woo
Kim Min-Ju
Kim Jung-Ok
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Abstract
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Objectives: To expand and provide information on the efficacy of herbal medicines, anti-obesity effects were evaluated. In many studies, plant-derived components with anti-obesity efficacies have been investigated for their potential inhibitory effects on 3T3-L1 preadipocyte cells. The purpose of this study was to investigate the anti-obesity effects of 65 herbal
medicine in 3T3-L1 preadipocyte cells.
Methods: Preferentially, 3T3-L1 cells were treated with 65 herbal medicines (500 μg/mL) during differentiation for 8 days. Next, 3T3-L1 cells were treated with selected herbal medicines at concentrations ranging from 50 to 200 μg/mL during differentiation for 8 days. The accumulation of lipid droplets was determined by Oil Red O staining. The expressions of genes related to adipogenesis were measured by reverse transcription polymerase chain reaction and Western blot analyses.
Results: Among the 65 kinds of herbal medicines, 13 herbal medicines that been shown to be effective against the accumulation of lipid droplets were selected. Finally, selected Banhasasim-tang and Samhwangsasim-tang showed inhibitory activity on adipocyte differentiation at 3T3-L1 preadipocytes without affecting cell toxicity. In addition, Banhasasim-tang and Samhwangsasim-tang significantly reduced the expression levels of several adipocyte marker genes including peroxisome proliferator activated receptor-γ and CCAAT/enhancer binding protein-α.
Conclusions : These results suggest that the ability of Banhasasim-tang and Samhwangsasimtang has inhibited overall adipogenesis and lipid accumulation in the 3T3-L1 cells.
Banhasasim-tang and Samhwangsasim-tang may be a promising medicine for the treatment of obesity and related metabolic disorders.
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KEYWORD
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Herbal medicine, obesity, 3T3-L1
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